Glow Discharge system for TEM grids.
HYDROPHILIC OR HYDROPHOBIC, NEGATIVE OR POSITIVE CHARGE
TEM carbon support films have a hydrophobic tendency.
A glow discharge treatment with air makes the carbon film surface negatively charged and hydrophilic which allows an adsorption of aqueous solutions.
Thanks to a glow discharge treatment with amylamine the hydrophobic tendency of the carbon film is kept and the surface is charged positively allowing an adsorption of molecules like nucleic acids.
BENEFITS
WITHOUT GLOW DISCHARGE
WITH GLOW DISCHARGE
Figure 1 : Negative staining with uranyl acetate of the viral capsids spread on a TEM grid without treatment.
Courtesy : M. Decossas, CBMN
Figure 2 : Negative staining with uranyl acetate of the Poliomyelite virus spread on a TEM grid with a glow discharge treatment with air (2mA during 40sec).
Courtesy : M. Decossas, CBMN
EASY
- Quick and easy loading of films or grids.
- Intuitive operation with manual or programmed mode.
- Affichage (courant, pression, durée) en temps réel des données sur écrans LCD rétrReal-time display of process parameters (current, vacuum, time).
FLEXIBLE
- Glow discharge methods : hydrophilic or hydrophobic,negative or positive charge.
- Dual vacuum Bell Jars to avoid cross contamination.
- Anti-implosion glass for an easy cleaning and a high secured process.
REPRODUCIBLE
- Accurate injection control of gaz or liquids.
- Accurate vacuum control using Pirani gauge.
- Soft venting (air or neutral gaz) using quick inlet fitting.
APPLICATIONS WITH GLOW DISCHARGE IN AMYLAMINE
Figure 3 : Negative staining with uranyl acetate and rotary shadowing with platinium of plasmid DNA.
Courtesy : C. Ruhlmann, IGBMC
Figure 4 : Negative staining with uranyl acetate and rotary shadowing with platinium of yeast chromatin.
Courtesy : C. Ruhlmann, IGBMC
APPLICATIONS WITH GLOW DISCHARGE IN AIR
Figure 5 : Negative staining with phosphotungstic acid of Influenza virus hemagglutinin.
Courtesy: M. Decossas, CBMN
Figure 6 : Negative staining with uranyl acetate of liposome and proteo-liposome.
Courtesy : L. Daury-Joucla, CBMN
Figure 7 : Torus obtained by complex coacervation between the peptide P140 and hyaluronic acid (therapeutic aim).
Courtesy : C. Blanck, ICS
ELMO Specifications
| Plasma current | 0 – 35 mA |
| Platform diameter | Ø 60 mm |
| Process time | 1 – 6000 seconds |
| Chamber size | Ø 80 mm x 60 mm H |
| Vacuum control | Pirani Gauge |
| Working vacuum range | 1,0 – 0,1 mbar |
| Operation mode | Manual & Programmed |
| Gaz inlets | Ø 6 mm |
| Instrument size | 480 x 310 x 320 mm |
| Weight | 12 kg |
Vacuum Pump Specifications
| Displacement 50/60 Hz | 3,7 m3h-1 / 4,5 m3h-1 |
| Speed (Pneurop 6602) 50/60 Hz | 3,3 m3h-1 / 3,9 m3h-1 |
| Ultimate pressure | 2,0 x 10-3 mbar |
| Motor power 50/60 Hz | 450 / 550 W |
| Power connector 1-ph | IEC EN60320 C13 |
| Nominal rotation speed 50/60 Hz | 1500/1800 rpm |
| Weight | 25 kg / 55 lb |
| Inlet / Exhaust flange | NW25 / NW25 |
| Noise level | 48 dBA à 50 Hz |
| Operating temperature range | 12 – 40 °C |
Notes d’application :
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